SNX18 Overexpression Lysate (Native)

Images

 
Western Blot: SNX18 Overexpression Lysate (Adult Normal) [NBL1-16309] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNX18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SNX18 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219150 and based on accession number NM_052870. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sorting nexin 18 (SNX18), transcript variant 2, mRNA.
Gene
SNX18

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SNX18

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SNX18 Overexpression Lysate (Native)

  • FLJ11997
  • FLJ61062
  • MGC150827
  • MGC150829
  • SH3 and PX domain-containing protein 3B
  • SH3PX2
  • SH3PXD3BFLJ32560
  • SNAG1
  • sorting nexin 18
  • sorting nexin associated golgi protein 1
  • sorting nexin-18
  • Sorting nexin-associated Golgi protein 1

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region compared to variant 1. The resulting protein (isoform b) has a longer and distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SNX18