Siglec-14 Antibody (2457E) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 2457E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

Siglec-14 Antibody (2457E) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human Siglec-14 in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-14 Antibody (2457E) [Alexa Fluor™ Plus 488]

• AC018755.2
• sialic acid binding Ig-like lectin 14
• Siglec14
• Siglec-14

Background

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V-set domain followed by a varying number of Ig-like C2-set domains (1, 2). Orthologs of Siglec-14 have not been described in mouse or rat. Mature human Siglec-14 consists of a 342 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and two Ig-like C2-set domains, a 23 aa transmembrane segment, and a 15 aa cytoplasmic tail (3). Within the first two Ig-like domains, Siglec-14 shares 99.5% aa sequence identity with Siglec-5, and 51% - 56% with Siglec-3, -6, -7, -8, and -9. Siglec-5 and -14 exhibit similar ligand preference among sialylated glycans, although Siglec-14 binds with higher avidity (3). Siglec-14 does not have the cytoplasmic ITIM sequence that mediates inhibitory signaling of most other Siglecs (2, 3). However, its transmembrane segment contains a charged arginine residue that enables association with the adaptor protein DAP12 (3, 4). Siglec-15 is the only other human Siglec identified to date that associates with DAP12 (5). Siglec-14 mRNA is primarily expressed in bone marrow, spleen, and fetal liver (3). The protein may be expressed on neutrophils, monocytes, and macrophages, as reports describing Siglec-5 on these cells employed some antibodies that also detect Siglec-14 (3, 6, 7). Siglec-5 and -14 likely function as paired receptors with similar ligand specificity and cellular expression but potentially opposing effects on cellular activation (3).

1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
3. Angata, T. et al. (2006) FASEB J. 20:1964.
4. Turnbull, I.R. and M. Colonna (2007) Nat. Rev. Immunol. 7:155.
5. Angata, T. et al. (2007) Glycobiology 17:838.
6. Connolly, N.P. et al. (2002) Br. J. Haematol. 119:221.
7. Erickson-Miller, C.L. et al. (2003) Exp. Hematol. 31:382.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.