Recombinant Human SF3B14 Protein

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SDS-Page: Recombinant Human SF3B14 Protein [NBP1-50997] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human SF3B14 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-125 of Human SF3B14

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SF3B14
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human SF3B14 Protein

  • HSPC175
  • Ht006
  • P14
  • pre-mRNA branch site protein p14
  • SAP14
  • SF3b 14 kDa subunit
  • SF3B14a
  • spliceosome-associated protein, 14 kDa subunit
  • splicing factor 3B, 14 kDa subunit

Background

SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SF3B14