Septin-2 Antibody (JB41-30)

Western Blot: Septin-2 Antibody (JB41-30) [NBP2-75660] - Analysis of Septin 2 on different cell lysates using anti-Septin 2 at 1/500 dilution.Positive control:Line 1: Mouse brain Line 2: SiHaLine 3: K562

Immunocytochemistry/Immunofluorescence: Septin-2 Antibody (JB41-30) [NBP2-75660] - Staining Septin 2 in SHSY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Septin-2 Antibody (JB41-30) [NBP2-75660] - Analysis of paraffin-embedded rat brain tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Septin-2 Antibody (JB41-30) [NBP2-75660] - Analysis of K562 cells with Septin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Septin-2 Antibody (JB41-30) [NBP2-75660] - Staining Septin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Septin-2 Antibody (JB41-30) [NBP2-75660] - Staining Septin 2 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Septin-2 Antibody (JB41-30) [NBP2-75660] - Analysis of paraffin-embedded human colon cancer tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Septin-2 Antibody (JB41-30) [NBP2-75660] - Analysis of paraffin-embedded human kidney tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.

Product Discontinued

Septin-2 Antibody (JB41-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Septin-2 aa 182-318 / 361. (SwissProt: Q15019 Human; SwissProt: P42208 Mouse; SwissProt: Q91Y81 Rat)
• Localization: Cytoplasm. Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SEPT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Septin-2 Antibody (JB41-30)

• DIFF6NEDD-5
• hNedd5
• KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
• NEDD5Pnutl3
• neural precursor cell expressed, developmentally down-regulated 5
• septin 2
• septin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPT2