Semaphorin 4F Antibody (780225)

Mouse cortical stem cells were stained with Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Semaphorin 4F was detected in perfusion fixed frozen sections of mouse spinal cord using Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to synaptic puncta. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 780225
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 4F Antibody (780225) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sema-4F
  Arg41-Gly667
  Accession # Q9Z123
• Specificity: Detects mouse Sema-4F in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SEMA4F
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 uL/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4F Antibody (780225)

• M-SEMA
• m-Sema-M
• PRO2353
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F
• Sema M
• Sema W
• SEMA4F
• SEMAM
• Semaphorin 4F
• semaphorin-4F
• semaphorin-M
• semaphorin-W
• SEMAW
• SEMAWsemaphorin M

Background

Semaphorin 4F (Sema4F; also Sema W) is a 90-100 kDa, class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively.

               

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 4F Antibody (MAB7200)(2)

Publications using MAB7200

• Simon G, Cecile F, Clemence O et al. TGF?-induced expression of long noncoding lincRNA Platr18 controls breast cancer axonogenesis. Life Sci Alliance. 2021-11-22 [PMID: 34810279]
• Simon G, Cecile F, Clemence O et al. TGF?-induced expression of long noncoding lincRNA Platr18 controls breast cancer axonogenesis. Life Sci Alliance. 2021-11-22 [PMID: 34810279]

Bioinformatics

• Gene Symbol: SEMA4F
• Uniprot:
  • Q9Z123(Mouse)