Selenoprotein W Antibody

Western Blot: Selenoprotein W Antibody [NBP1-49599] - Western blot using Selenoprotein W antibody antibody shows detection of enCanineenous Selenoprotein W in mouse brain, muscle and testes lysates. Recombinant Selenoprotein W is also detected at 10 ng (right lanes). The arrow corresponds with Selenoprotein W protein at 9.6 kDa. The primary antibody was used at a 1:1000 dilution.

N/A: Selenoprotein W Antibody [NBP1-49599]

Product Discontinued

Selenoprotein W Antibody Summary

• Immunogen: This affinity purified Selenoprotein W Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full-length mouse Selenoprotein W protein. (Uniprot: P63300)
• Predicted Species: Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPW1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100000
  • Immunoprecipitation 1:100
  • Western Blot 1:1000-1:5000
• Application Notes: Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.6 kDa in size corresponding to Selenoprotein W by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both overexpressed and endogenous Selenoprotein W.
• Theoretical MW: 9.54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Selenoprotein W from rat based on a 98% homology with the immunizing sequence. Partial reactivity is expected against human-derived Selenoprotein W based on an 87% homology with the immunogen. Cross-reactivity with Selenoprotein W from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Selenoprotein W Antibody

• selenoprotein W
• selenoprotein W, 1
• SELW

Background

Selenoprotein W was first purified and characterized from rat skeletal muscle. The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation-reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Selenoprotein W Antibody (NBP1-49599)(1)

Publication using NBP1-49599

• Shin MG, Cha HN, Park S et al. Selenoprotein W Deficiency Does Not Affect Oxidative Stress and Insulin Sensitivity in the Skeletal Muscle of High-fat Diet-fed Obese Mice Am. J. Physiol., Cell Physiol. 2019-09-11 [PMID: 31509445]

Bioinformatics

• Gene Symbol: SEPW1