Selenoprotein W Antibody

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Western Blot: Selenoprotein W Antibody [NBP1-49599] - Analysis of endogenous SelW in mouse brain, muscle and testes lysates. Recombinant SelW is also detected at 10 ng (right lanes). The arrow corresponds with SelW ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Selenoprotein W Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full-length mouse SelW protein.
Specificity
This antibody is specific for mouse Selenoprotein W. A BLAST analysis was used to suggest cross-reactivity with SelW from rat based on a 98% identity with the immunizing sequence. Partial reactivity is expected against human-derived SelW based on an 87% homology with the immunogen. Cross-reactivity with SelW from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPW1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:100000
  • Immunoprecipitation 1:100
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 9.6 kDa in size corresponding - SelW by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both overexpressed and endogenous SelW.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Selenoprotein W Antibody

  • selenoprotein W
  • selenoprotein W, 1
  • SELW

Background

Selenoprotein W was first purified and characterized from rat skeletal muscle. The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation-reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SEPW1