Sec8 Antibody (4F1) - Azide and BSA Free

Western Blot: Sec8 Antibody (4F1) [H00060412-M06] - Analysis of EXOC4 expression in Raw 264.7 (Cat # L024V1).

Western Blot: Sec8 Antibody (4F1) [H00060412-M06] - Analysis of EXOC4 expression in PC-12 (Cat # L012V1).

Western Blot: Sec8 Antibody (4F1) [H00060412-M06] - Analysis of EXOC4 expression in Hela S3 NE (Cat # L013V3).

Western Blot: Sec8 Antibody (4F1) [H00060412-M06] - Analysis of EXOC4 expression in NIH/3T3 (Cat # L018V1).

ELISA: Sec8 Antibody (4F1) [H00060412-M06] - Detection limit for recombinant GST tagged EXOC4 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 4F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Sec8 Antibody (4F1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Sec8 Antibody (4F1) - Azide and BSA Free (H00060412-M06) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-Sec8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: EXOC4 (NP_068579, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELTTAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRD
• Specificity: EXOC4 (4F1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EXOC4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Sec8 Antibody (4F1) - Azide and BSA Free

• exocyst complex component 4
• KIAA1699SEC8L1
• MGC27170
• REC8
• SEC8Exocyst complex component Sec8
• SEC8-like 1 (S. cerevisiae)
• SEC8-like 1
• Sec8p

Background

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sec8 Antibody (H00060412-M06)(1)

Publication using H00060412-M06

• Imai A, Yoshie S, Haga-Tsujimura M et al. Exocyst subunits are involved in isoproterenol-induced amylase release from rat parotid acinar cells. Eur J Oral Sci. 2012-04-01 [PMID: 22409218]

Bioinformatics

• Gene Symbol: EXOC4
• Entrez:
  • 60412(Human)
• OMIM:
  • 608185(Human)
• Uniprot:
  • NP_068579(Human)