SEC6 Antibody (4A7) - Azide and BSA Free

Western Blot: SEC6 Antibody (4A7) [H00011336-M01] - Analysis of EXOC3 expression in transfected 293T cell line by EXOC3 monoclonal antibody (M01), clone 4A7.Lane 1: EXOC3 transfected lysate(86.845 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: SEC6 Antibody (4A7) [H00011336-M01] - Detection limit for recombinant GST tagged EXOC3 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 4A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SEC6 Antibody (4A7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SEC6 Antibody (4A7) - Azide and BSA Free (H00011336-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-SEC6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: EXOC3 (NP_009208, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK
• Specificity: EXOC3 (4A7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EXOC3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SEC6 Antibody (4A7) - Azide and BSA Free

• exocyst complex component 3
• Exocyst complex component Sec6
• Sec 6 homolog
• SEC6
• SEC6L1
• SEC6-like 1 (S. cerevisiae)
• SEC6-like 1
• Sec6p

Background

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEC6 Antibody (H00011336-M01)(2)

Publications using H00011336-M01

• Raghavan B, Ng ML. West nile virus and Dengue virus capsid protein negates the antiviral activity of human Sec3 protein Through The Proteasome Pathway. Cell Microbiol. 2013-03-22 [PMID: 23522008]
• Imai A, Yoshie S, Haga-Tsujimura M et al. Exocyst subunits are involved in isoproterenol-induced amylase release from rat parotid acinar cells. Eur J Oral Sci. 2012-04-01 [PMID: 22409218]

Bioinformatics

• Gene Symbol: EXOC3
• Entrez:
  • 11336(Human)
• OMIM:
  • 608186(Human)
• Uniprot:
  • NP_009208(Human)