SEC31A Antibody - Azide and BSA Free

Western Blot: SEC31A Antibody [NBP2-93172] - Analysis of extracts of various cell lines, using SEC31A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.

Immunocytochemistry/ Immunofluorescence: SEC31A Antibody - Azide and BSA Free [NBP2-93172] - Immunofluorescence analysis of HepG2 cells using SEC31A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Product Discontinued

SEC31A Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human SEC31A (NP_055748.2). PTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSTDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQTAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFENVRMPSHQGAEQQQQQHHVFISQVVTEKEFLSRSDQLQQAVQSQGFINYCQKKIDASQTEFEKNVWSFLKVNFEDDSRGKYLELLGYRKEDL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEC31A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:1000 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for SEC31A Antibody - Azide and BSA Free

• ABP125ABP130KIAA0905Web1-like protein
• DKFZp686N07171
• HSPC334
• MGC90305
• protein transport protein Sec31A
• protein-transport protein SEC31
• SEC31 homolog A (S. cerevisiae)
• SEC31L1
• SEC31-like 1 (S. cerevisiae)
• SEC31-like 1
• SEC31-like protein 1
• SEC31-related protein A
• yeast Sec31p homolog

Background

Sec31A is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SEC31A