RPL35A Overexpression Lysate (Native)

Images

 
Western Blot: RPL35A Overexpression Lysate (Adult Normal) [NBL1-15531] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPL35A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RPL35A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203131 and based on accession number NM_000996. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ribosomal protein L35a (RPL35A), mRNA.
Gene
RPL35A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RPL35A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RPL35A Overexpression Lysate (Native)

  • Cell growth-inhibiting gene 33 protein
  • DBA5,60S ribosomal protein L35a
  • ribosomal protein L35a

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Transcript variants utilizing alternative transcription initiation sites and alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006223-M01
Species: Hu
Applications: WB, ELISA
NBP2-20210
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-31413
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00006218-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P
NBP1-81330
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80803
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00006201-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
H00006193-M02
Species: Hu, Mu, Rt, Pr
Applications: WB, ELISA, ICC/IF
NBP1-31357
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-30682
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-92352
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2277
Species: Hu, Mu
Applications: WB, IHC-P, IP
H00009768-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-20216
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1595
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP2-33717
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB100-2269
Species: Hu, Mu
Applications: WB, IHC-P, IP
H00006143-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBL1-15531
Species: Hu
Applications: WB

Publications for RPL35A Lysate (NBL1-15531) (0)

There are no publications for RPL35A Lysate (NBL1-15531).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RPL35A Lysate (NBL1-15531) (0)

There are no reviews for RPL35A Lysate (NBL1-15531). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RPL35A Lysate (NBL1-15531) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RPL35A Products

Bioinformatics Tool for RPL35A Lysate (NBL1-15531)

Discover related pathways, diseases and genes to RPL35A Lysate (NBL1-15531). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RPL35A Lysate (NBL1-15531)

Discover more about diseases related to RPL35A Lysate (NBL1-15531).
 

Pathways for RPL35A Lysate (NBL1-15531)

View related products by pathway.

PTMs for RPL35A Lysate (NBL1-15531)

Learn more about PTMs related to RPL35A Lysate (NBL1-15531).

Blogs on RPL35A

There are no specific blogs for RPL35A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

RPL7 Antibody
NB100-2269
RPL35A RNAi
H00006165-R01-20nmol
Bcl-xL Antibody
NB100-56104

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RPL35A Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RPL35A