![Western Blot: RNPEPL1 Antibody (4A9) [NBP2-59371] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 (SMC-263). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-263) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.](http://images.novusbio.com/fullsize/RNPEPL1-Antibody-4A9-Western-Blot-NBP2-59371-img0001.jpg "Western Blot: RNPEPL1 Antibody (4A9) [NBP2-59371] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 (SMC-263). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-263) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, Flow |
| Clone | 4A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Mouse RNPEPL1 Antibody (4A9) - BSA Free (NBP2-59371) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic N-terminal arginylated peptide conjugated to KLH |
| Localization | Endoplasmic Reticulum |
| Specificity | Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNPEPL1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RNPEPL1 |
![Flow Cytometry: RNPEPL1 Antibody (4A9) [NBP2-59371] - Flow Cytometry analysis using Mouse Anti-RNPEPL1 Monoclonal Antibody, Clone 4A9 (NBP2-59371). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-RNPEPL1 Monoclonal Antibody (NBP2-59371) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42C for 2 hours.](http://images.novusbio.com/fullsize/RNPEPL1-Antibody-4A9-Flow-Cytometry-NBP2-59371-img0002.jpg "Flow Cytometry: RNPEPL1 Antibody (4A9) [NBP2-59371] - Flow Cytometry analysis using Mouse Anti-RNPEPL1 Monoclonal Antibody, Clone 4A9 (NBP2-59371). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-RNPEPL1 Monoclonal Antibody (NBP2-59371) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42C for 2 hours.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")