RIC8A Antibody (OTI3G3) - Azide and BSA Free

Western Blot: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8.

Immunocytochemistry/Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8.

Immunohistochemistry: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of paraffin-embedded Kidney tissue using anti-RIC8 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: RIC8A Antibody (OTI3G3) - Azide and BSA Free [NBP2-73908] - Staining of HepG2 cells using anti-RIC8 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RIC8A Antibody (OTI3G3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RIC8A Antibody (OTI3G3) - Azide and BSA Free (NBP2-02433) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIC8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 59.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RIC8A Antibody (OTI3G3) - Azide and BSA Free

• RIC8
• RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• synembryn
• synembryn-A

Background

RIC8 is a likely orthalog of mouse Synembryn. Synembryns are proteins that positively regulate synaptic transmission. They are actively required to maintain proper activity of the Go to Gq G-protein signalling network, which regulates neurotransmitter secretion in Caenorhabditis elegans by controlling the production and consumption of diacylglycerol. In addition to its role in the adult nervous system, synembryn is required to regulate a subset of centrosome movements in the early C. elegans embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIC8A