RIC8A Antibody (OTI3G3)

Western Blot: RIC8 Antibody (3G3) [NBP2-02433] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8.

Immunocytochemistry/Immunofluorescence: RIC8 Antibody (3G3) [NBP2-02433] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8.

Immunohistochemistry-Paraffin: RIC8 Antibody (3G3) [NBP2-02433] - Staining of paraffin-embedded Kidney tissue using anti-RIC8 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: RIC8 Antibody (3G3) [NBP2-02433] - Immunofluorescent staining of HepG2 cells using anti-RIC8 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.83 mg/ml

RIC8A Antibody (OTI3G3) Summary

• Description: Novus Biologicals Mouse RIC8A Antibody (OTI3G3) (NBP2-02433) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIC8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 59.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.83 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RIC8A Antibody (OTI3G3)

• RIC8
• RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• synembryn
• synembryn-A

Background

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIC8A
• Entrez:
  • 60626(Human)