RhoB Antibody - Azide and BSA Free

Product Discontinued

RhoB Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide as a part of human, rat, mouse RhoB conjugated to an immunogenic carrier protein was used as the immunogen.
• Localization: subcellular location: late endosome membrane; lipid-anchor. cell membrane; lipid-anchor. nucleus. note=late endosomal membrane (geranylgeranylated form). plasma membrane (farnesylated form). also detected at the nuclear margin and in the nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHOB
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Application Notes: Western Blot. By Western blot, the MW of the detected band is ~25 kDa. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen.
• Control:

  RhoB Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for RhoB Antibody - Azide and BSA Free

• ARH6MSTP081
• ARHBAplysia RAS-related homolog 6
• h6
• MST081
• oncogene RHO H6
• ras homolog gene family, member B
• Rho cDNA clone 6
• RHOH6RhoB
• rho-related GTP-binding protein RhoB

Background

Mediates apoptosis in neoplastically transformed cells after dna damage. not essential for development but affects cell adhesion and growth factor signaling in transformed cells. plays a negative role in tumorigenesis as deletion causes tumor formation. involved in intracellular protein trafficking of a number of proteins. targets pkn1 to endosomes and is involved in trafficking of the egf receptor from late endosomes to lysosomes. also required for stability and nuclear trafficking of akt1/akt which promotes endothelial cell survival during vascular development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RhoB Antibody (NBP1-03484)(1)

Publication using NBP1-03484

• Saito M, Ohmasa F, Shomori K et al. Rhos and Rho kinases in the rat prostate: their possible functional roles and distributions. Mol Cell Biochem. 2011-07-01 [PMID: 21720764] (WB, IF/IHC, Rat)

FAQs for RhoB Antibody (NBP1-03484). (Showing 1 - 1 of 1 FAQ).

1. I just ordered this antibody and when I opened the vial, I found it contains of something a large like impurity visibly. Do you know what this is?
   • This product is supplied as lyophilized powder. You will need to centrifuge the vial and reconstitute the lyophilized antibody in 0.5ml of sterile dH2O. We also recommend that you centrifuge to remove any insoluble material after reconstitution. When reconstituting, glycerol (1:1) may be added for an additional stability.

Bioinformatics

• Gene Symbol: RHOB
• Entrez:
  • 388(Human)
  • 11852(Mouse)
  • 64373(Rat)
• Uniprot:
  • P62745(Human)