RhoB Antibody

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

RhoB Antibody Summary

Immunogen
A synthetic peptide as a part of human, rat, mouse Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6) conjugated to an immunogenic carrier protein was used as the immunogen.
Localization
subcellular location: late endosome membrane; lipid-anchor. cell membrane; lipid-anchor. nucleus. note=late endosomal membrane (geranylgeranylated form). plasma membrane (farnesylated form). also detected at the nuclear margin and in the nucleus.
Specificity
RhOB.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 10-50 ug/ml
  • Immunohistochemistry 1:10-1:500
Application Notes
Western Blot. By Western blot, the MW of the detected band is ~25 kDa. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen.
Positive Control
RhoB Lysate (NBL1-15350)
Publications
Read Publication using
NBP1-03484 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Reacts with Human, Rat and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized water. Add glycerol (1:1) for additional stability.

Alternate Names for RhoB Antibody

  • ARH6MSTP081
  • ARHBAplysia RAS-related homolog 6
  • h6
  • MST081
  • oncogene RHO H6
  • ras homolog gene family, member B
  • Rho cDNA clone 6
  • RHOH6RhoB
  • rho-related GTP-binding protein RhoB

Background

Mediates apoptosis in neoplastically transformed cells after dna damage. not essential for development but affects cell adhesion and growth factor signaling in transformed cells. plays a negative role in tumorigenesis as deletion causes tumor formation. involved in intracellular protein trafficking of a number of proteins. targets pkn1 to endosomes and is involved in trafficking of the egf receptor from late endosomes to lysosomes. also required for stability and nuclear trafficking of akt1/akt which promotes endothelial cell survival during vascular development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoB Antibody (NBP1-03484)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
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Filter By Species
Rat
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RhoB Antibody (NBP1-03484). (Showing 1 - 1 of 1 FAQ).

  1. I just ordered this antibody and when I opened the vial, I found it contains of something a large like impurity visibly. Do you know what this is?
    • This product is supplied as lyophilized powder. You will need to centrifuge the vial and reconstitute the lyophilized antibody in 0.5ml of sterile dH2O. We also recommend that you centrifuge to remove any insoluble material after reconstitution. When reconstituting, glycerol (1:1) may be added for an additional stability.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for RhoB Antibody (NBP1-03484)

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PTMs for RhoB Antibody (NBP1-03484)

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Bioinformatics

Gene Symbol RHOB
Entrez
Uniprot