RGS8 Antibody

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Western blot shows lysates of human brain (Alzheimer's disease cortex) tissue and human brain (normal cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RGS8 Antigen Affinity-purified Polyclonal ...read more
RGS8 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human RGS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6880) at 3 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RGS8 Antibody Summary

Immunogen
E. coli-derived recombinant human RGS8
Asn10-Thr76
Accession # P57771
Specificity
Detects human RGS8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human RGS4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
RGS8
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGS8 Antibody

  • MGC119067
  • MGC119068
  • MGC119069
  • regulator of G-protein signaling 8
  • regulator of G-protein signalling 8
  • RGS8

Background

RGS8 (Regulator of G-protein signaling 8) is a 22-23 kDa (predicted) member of the B/R4 subfamily, RGS family of proteins. It is expressed in NK cells and neurons, particularly cerebellar Purkinje cells, and is known to regulate GPCR signaling. When GPCRs are activated, a GPCR cytoplasmic domain associated G alpha beta gamma heterotrimer dissociates in response to GTP binding. This generates G alpha :GTP and G beta gamma dimers that initiate downstream signaling. G alpha has GTPase activity, and when GTP is hydrolyzed, the G alpha beta gamma trimer reforms and stops signaling. RGS8 potentiates G alpha GTP hydrolase activity, particularly when associated with M1 muscarinic acetylcholine receptors. Human RGS8 is 180 amino acids (aa) in length, and is characterized by the presence of one RGS domain (aa 56-171), and an N-terminal GPCR binding motif (aa 6-9). There is one splice variant that shows a 27 aa substitution for aa 1-9. Expression of this variant may negatively impact its regulation of G alpha -coupled responses. Over aa 10-76, human RGS8 shares 98% aa identity with mouse RGS8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on RGS8.

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.

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Bioinformatics

Gene Symbol RGS8
Uniprot