RGS8 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

RGS8 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human RGS8
  Asn10-Thr76
  Accession # P57771
• Specificity: Detects human RGS8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human RGS4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGS8 Antibody [Alexa Fluor® 405]

• MGC119067
• MGC119068
• MGC119069
• regulator of G-protein signaling 8
• regulator of G-protein signalling 8
• RGS8

Background

RGS8 (Regulator of G-protein signaling 8) is a 22-23 kDa (predicted) member of the B/R4 subfamily, RGS family of proteins. It is expressed in NK cells and neurons, particularly cerebellar Purkinje cells, and is known to regulate GPCR signaling. When GPCRs are activated, a GPCR cytoplasmic domain associated G alpha beta gamma heterotrimer dissociates in response to GTP binding. This generates G alpha :GTP and G beta gamma dimers that initiate downstream signaling. G alpha has GTPase activity, and when GTP is hydrolyzed, the G alpha beta gamma trimer reforms and stops signaling. RGS8 potentiates G alpha GTP hydrolase activity, particularly when associated with M1 muscarinic acetylcholine receptors. Human RGS8 is 180 amino acids (aa) in length, and is characterized by the presence of one RGS domain (aa 56-171), and an N-terminal GPCR binding motif (aa 6-9). There is one splice variant that shows a 27 aa substitution for aa 1-9. Expression of this variant may negatively impact its regulation of G alpha -coupled responses. Over aa 10-76, human RGS8 shares 98% aa identity with mouse RGS8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.