RGS3 Overexpression Lysate

Images

 
Western Blot: RGS3 Overexpression Lysate (Adult Normal) [NBL1-15333] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RGS3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RGS3 Overexpression Lysate Summary

Description
Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC224058 and based on accession number NM_130795. The protein contains a C-MYC/DDK Tag.
Gene
RGS3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
100.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RGS3 Overexpression Lysate

  • C2PA
  • FLJ20370
  • FLJ31516
  • FLJ90496
  • PDZ-RGS3
  • regulator of G-protein signaling 3
  • regulator of G-protein signalling 3
  • RGP3

Background

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTP-ase activating protein which inhibits G-protein mediated signal transduction. The protein is largely cytosolic, but G-protein activation leads to translocation of this protein to the plasma membrane. A nuclear form of this protein has also been described, but its sequence has not been identified. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature of some transcripts is not yet known. Transcript Variant: This variant (1), also known as PDZ-RGS3, contains an alternate 5' coding region, as compared to variant 6. Isoform 1 has a shorter and distinct N-terminus compared to isoform 6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RGS3 Lysate (NBL1-15333) (0)

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-15333

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Bioinformatics

Gene Symbol RGS3