RGS16 Antibody - BSA Free

Western Blot: RGS16 Antibody [NB300-350] - E coli-derived fusion protein as test antigen. Affi-anti- RGS16 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS16 )

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RGS16 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken RGS16 Antibody - BSA Free (NB300-350) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RGS16 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide: corresponding to amino acids 130-202 of Human RGS16 (NP_002919.1).
• Specificity: RGS16
• Clonality: Polyclonal
• Host: Chicken
• Gene: RGS16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:200-1:500
• Application Notes: ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry reported in scientific literature (PMID: 23858031) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23858031)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat..Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for RGS16 Antibody - BSA Free

• A28-RGS14
• A28-RGS14P
• hRGS-r
• regulator of G-protein signaling 16
• regulator of G-protein signalling 16
• Retinally abundant regulator of G-protein signaling
• Retinal-specific RGS
• RGSR
• RGS-r

Background

RGS16 (regulator of G protein signalling 16) negatively regulates G protein coupled receptor signalling. RGS16 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP bound form. Binds to G(i) alpha and G(o) alpha, but not to G(s) alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS16 Antibody (NB300-350)(1)

Publication using NB300-350

• Ding Y, Li J, Wu Q et al. IL-17RA Is Essential for Optimal Localization of Follicular Th Cells in the Germinal Center Light Zone To Promote Autoantibody-Producing B Cells. J Immunol 2013-08-15 [PMID: 23858031] (IHC-Fr, ICC/IF, WB, Mouse)

Bioinformatics

• Gene Symbol: RGS16
• Entrez:
  • 6004(Human)
  • 19734(Mouse)
  • 360857(Rat)
• Uniprot:
  • O15492(Human)