RENT1/UPF1/hUPF1 Antibody (JG38-44)

Western Blot: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - RENT1/UPF1/Hupf1 Antibody (JG38-44) [NBP2-76842] - ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.

Flow Cytometry: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - RENT1/UPF1/Hupf1 Antibody (JG38-44) [NBP2-76842] - ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - RENT1/UPF1/Hupf1 Antibody (JG38-44) [NBP2-76842] - ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RENT1/UPF1/hUPF1 Antibody (JG38-44) [NBP2-76842] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.

Product Discontinued

RENT1/UPF1/hUPF1 Antibody (JG38-44) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human RENT1/UPF1/hUPF1 aa 1-120 / 1129. (SwissProt: Q92900 Human; SwissProt: Q9EPU0 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UPF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RENT1/UPF1/hUPF1 Antibody (JG38-44)

• ATP-dependent helicase RENT1
• EC 3.6.1
• EC 3.6.4.-
• FLJ43809
• HUPF1
• KIAA0221FLJ46894
• Nonsense mRNA reducing factor 1
• NORF1
• NORF1delta helicase
• pNORF1
• regulator of nonsense transcripts 1
• RENT1
• RENT1UP Frameshift 1
• UPF1 regulator of nonsense transcripts homolog (yeast)
• UPF1
• up-frameshift mutation 1 homolog
• Up-frameshift suppressor 1 homolog
• yeast Upf1p homolog

Background

hUPF1 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UPF1