Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

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Product Details

Summary
Reactivity YeSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF Summary

Additional Information
Will be discontinued when existing inventory is gone.
Details of Functionality

Recombinant Yeast GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.   We recommend an initial GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived yeast Ubiquitin-activating Enzyme/UBE1 protein
Met1 - Leu1024 with a N-terminal GST (glutathione S-transferase) tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBA1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-300 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

  • A1S9T and BN75 temperature sensitivity complementing
  • A1S9T
  • A1ST
  • AMCX1
  • GXP1
  • MGC4781
  • POC20 centriolar protein homolog
  • POC20
  • Protein A1S9
  • SMAX2
  • UBA1
  • UBA1, ubiquitin-activating enzyme E1 homolog A
  • UBA1A
  • UBE1
  • UBE1A1S9
  • UBE1X
  • ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
  • Ubiquitin-activating enzyme E1
  • Ubiquitinactivating Enzyme
  • Ubiquitin-activating Enzyme
  • ubiquitin-like modifier activating enzyme 1
  • ubiquitin-like modifier-activating enzyme 1

Background

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1024 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 114 kDa.  Human and mouse UBE1 share 52% and 53% aa sequence identity with the yeast UBE1 protein, respectively.  UBE1 is found in the cytoplasm and nucleus, and contains a conserved active site cysteine residue and ATP-binding site common to E1 enzymes (1-3).  UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5).  Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys600 in S. cerevisiae, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8).  The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating-Ubiquitin ligase enzyme cascade.  UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.  
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. and J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. and I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. and P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.

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Publications for Ubiquitin-activating Enzyme/UBE1 (E-300)(6)

We have publications tested in 3 confirmed species: N/A, Plant, Yeast.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
All Applications
Filter By Species
N/A
(3)
Plant
(1)
Yeast
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using E-300 Applications Species
Singh, S;Yeat, NY;Wang, YT;Lin, SY;Kuo, IY;Wu, KP;Wang, WJ;Wang, WC;Su, WC;Wang, YC;Chen, RH; PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target Cell death & disease 2023-10-11 [PMID: 37821451] (Bioassay, N/A) Bioassay N/A
Radmall, KS;Shukla, PK;Leng, AM;Chandrasekharan, MB; Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains Scientific reports 2023-10-04 [PMID: 37794081] (Bioassay, N/A) Bioassay N/A
Xu, W;Yu, Y;Jing, J;Wu, Z;Zhang, X;You, C;Ma, H;Copenhaver, GP;He, Y;Wang, Y; SCFRMF mediates degradation of the meiosis-specific recombinase DMC1 Nature communications 2023-08-19 [PMID: 37598222] (Bioassay, Plant) Bioassay Plant
PK Shukla, JE Bissell, S Kumar, S Pokhrel, S Palani, KS Radmall, O Obidi, TJ Parnell, J Brasch, DC Shrieve, MB Chandrasek Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex Nucleic Acids Research, 2023-03-21;0(0):. 2023-03-21 [PMID: 36715322] (Bioassay, N/A) Bioassay N/A
PK Shukla, D Sinha, AM Leng, JE Bissell, S Thatipamul, R Ganguly, KS Radmall, JJ Skalicky, DC Shrieve, MB Chandrasek Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 affect ubiquitination activity and decrease enzyme stability The Journal of Biological Chemistry, 2022-09-23;0(0):102524. 2022-09-23 [PMID: 36162503] (Bioassay, Yeast) Bioassay Yeast
S Lee, JM Tumolo, AC Ehlinger, KK Jernigan, SJ Qualls-His, PC Hsu, WH McDonald, WJ Chazin, JA MacGurn Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin Elife, 2017-11-13;6(0):. 2017-11-13 [PMID: 29130884] (Bioassay, Yeast) Bioassay Yeast

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Bioinformatics

Gene Symbol UBA1
Uniprot