Recombinant Mouse VSTM4 His-tag Protein, CF

Recombinant Mouse VSTM4 (Catalog # 10210-VT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-5 μg/mL.

2 μg/lane of Recombinant Mouse VSTM4 His-tag (Catalog # 10210-VT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-44 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSTM4 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.5-5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSTM4 protein
  Leu24-Tyr179, with C-terminal 6-His tag
• Accession #: T1NXB5
• N-terminal Sequence: Leu24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-44 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSTM4 His-tag Protein, CF

• C10orf72
• FLJ31737
• hypothetical protein LOC196740
• V-set and transmembrane domain containing 4
• VSTM4

Background

V-set and transmembrane domain-containing protein 4 (VSTM4) is a single-pass type I membrane protein in the immunoglobulin superfamily. Mouse VSTM4 is synthesized as a 319 amino acid (aa) precursor that contains a 23 aa signal sequence, a 156 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 119 aa cytoplasmic tail. In humans, part of the extracellular region is cleaved into a 50 aa secreted peptide (aa 55-104) compared to mouse, which is cleaved into a 49 aa peptide (aa 55-103) (1). Because of its role in enhancing L-type voltage-gated calcium channel (L-VGCC) currents in photoreceptors, this peptide was named peptide Lv (1). Peptide Lv is expressed in the central nervous system and a variety of organs including spleen, intestine, retina, and lung (1, 2). The peptide may have possible roles in regulating the cardiovascular system and L-VGCC dependent neural plasticity (1, 2). Human VSTM4 gene is located on chromosome 10, which may be linked to late-onset Alzheimer's disease (3). Down-regulation of VSTM4 increased tamoxifen sensitivity and suppressed growth in cultured breast cancer cells (4). Within the ECD, mouse VSTM4 shares 87% and 96% aa sequence identity with human and rat VSTM4, respectively. The biological functions of VSTM4 remain unknown. Our in-house data show that mouse VSTM4 inhibits anti-CD3 induced IL-2 secretion by Human T Cells.

1. Shi, L. et al. (2012) PLoS. 7:e43091.
2. Shi, L. et al. (2015) Biochim. Biophys. Acta. 1853:1154.
3. Grupe, A. et al. (2006) Am. J. Hum. Genet. 78:78.
4. Mendes-Pereira, A. et al. (2012) Proc. Natl. Acad. Sci. 109:2730.

Bioinformatics

• Uniprot:
  • T1NXB5()