Recombinant Mouse SEZ6/BSRP-C Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SEZ6/BSRP-C Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells. 2 μg/mL of Recombinant Mouse SEZ6/BSRP-C is able to signifcantly induce cell clumping and inhibit neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse SEZ6/BSRP-C protein
  Gly19-Asn902, with a C-terminal 6-His tag
• Accession #: Q7TSK2
• N-terminal Sequence: Gly19
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sez6
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 140-190 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SEZ6/BSRP-C Protein, CF

• BSRPC
• BSRP-C
• hSEZ-6
• seizure related 6 homolog (mouse)
• SEZ6

Background

Seizure Related Gene 6 (SEZ6), also known as Brain-specific Receptor-like Protein C (BSRP-C), is a member of the SEZ6 family of proteins with a predicted molecular weight of 150 kDa (1, 2). It is a type I transmembrane glycoprotein composed of an extracellular domain that contains three CUB domains and five short consensus repeats (SCRs) (1-3). Alternative splicing generates two additional isoforms for SEZ6. One isoform is a transmembrane protein that shows a deletion of amino acids (aa) 895-907 plus a 10 aa substitution for aa 982-991. The second splice variant is a soluble protein that shows an 18 aa substitution for aa 588-991 (1, 2). Mouse SEZ6 shares 91% and 97% aa sequence identity with the human and rat orthologs, respectively. SEZ6 is expressed in neurons in the developing and adult mouse brain and retina (1-6). It has been suggested that BSRP-C is important for proper development of dendritic arbors and excitatory synapses. SEZ6 has been shown to inhibit neurite branching of cortical neurons with little effect on neurite extension (7). Additionally, SEZ6 has been shown to interact with Motopsin/PRSS12, a serine protease believed to be essential for the reorganization of neuronal structures in the developing and adult brain (8). Mutations in Sez6 have been associated with febrile seizures (9, 10).

1. Shimizu-Nishikawa, K. et al. (1995) Biochem. Biophys. Res. Commun. 216:382.
2. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
3. Shimizu-Nishikawa, K. et al. (1995) Brain Res. Mol. Brain Res. 28:201.
4. Kim, M.H. et al. (2002) Mech. Dev. 118:171.
5. Gunnersen, J.M. et al. (2009) PLoS One 4:e6546.
6. Osaki, G. et al. (2011) Brain Res. 1386:58.
7. Gunnersen, J.M. et al. (2007) Neuron 56:621.
8. Mitsui, S. et al. (2013) Biochem. Biophys. Res. Commun. 436:638.
9. Yu, Z.L. et al. (2007) J. Neurosci. Res. 85:166.
10. Mulley, J.C. et al. (2011) Neurol. Res. Int. 2011:917565.

Bioinformatics

• Gene Symbol: Sez6
• Uniprot:
  • Q7TSK2()