Recombinant Mouse PLA2G2A Protein, CF


There are currently no images for PLA2G2A (4925-PL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Mouse PLA2G2A Protein, CF Summary

Details of Functionality
Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine. The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse PLA2G2A protein
Asn22-Cys146, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 50 mM Tris, 500 mM NaCl, 1 mg/mL BSA, pH 8.5.
  • Substrate Buffer: 50 mM Tris, 500 mM NaCl, 20 mM CaCl2, 1 mg/mL BSA, pH 8.5.
  • Recombinant Mouse Phospholipase A2 Group IIA/PLA2G2A (rmPLA2G2A) (Catalog # 4925-PL)
  • Substrate: 1-hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine (Invitrogen, Catalog # H361), Dilute to 2 mM in DMSO, then dilute to a final stock concentration of 400 µM in ethanol
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Thaw Substrate at 37 °C for five minutes. Mix well.
  2. Dilute rmPLA2G2A to 2 ng/µL in Assay Buffer.
  3. Dilute substrate PGPC to 20 µM in Substrate Buffer.
  4. Load in plate 50 µL of 2 ng/µL rmPLA2G2A, and start the reaction by adding 50 µL of 20 µM Substrate to each well. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL Substrate.
  5. Read at excitation and emission wavelengths of 345 nm and 395 nm (top read), respectively, in kinetic mode for 5 minutes. For good linearity, shake the plate for 3 seconds in between each read.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 1-pyrenedecanoic acid (Invitrogen, Catalog # P31).

Per Well:
  • rmPLA2G2A: 0.1 µg
  • Substrate: 10 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PLA2G2A Protein, CF

  • EC
  • GIIC sPLA2
  • Group IIA phospholipase A2
  • Mom1
  • Non-pancreatic secretory phospholipase A2
  • NPS-PLA2
  • Phosphatidylcholine 2-acylhydrolase 2A
  • phospholipase A2, group IIA (platelets, synovial fluid)
  • phospholipase A2, membrane associated
  • PLA2B
  • PLA2G2A
  • PLA2L
  • PLAS1
  • RASF-A
  • sPLA2


Secretory Phospholipase A2 is an enzyme that hydrolyses the sn-2 ester bond of phospholipids, generating non-esterified free fatty acids and lysophospholipids (1‑3). PLA2G2A is a calcium-dependent phospholipase expressed in many cell types participating in inflammation-associated cellular responses, including platelets, neutrophils, and mast cells. It may function as an enzymatic component of the host defense mechanism. For example, human tears contain a high concentration of PLA2G2A, a principal bactericidal factor against Gram-positive bacteria in this fluid. It may play a role in cell proliferation through binding a receptor on the cell membrane. PLA2G2A has been shown to have pro-atherogenic properties both in the circulation and within the arterial wall (4). It is an acute phase protein expressed in response to a variety of pro-inflammatory cytokines. Circulating levels of sPLA2G2A are higher in coronary artery disease (CAD) patients and are associated with increased risk of future CAD (5).

  1. Webb, N. R. (2005) Cur. Opin. Lipid. 16:341.
  2. Triggiani, M. et al. (2005) J. Allergy Clin. Immunol. 116:1000.
  3. Murakami, M. and Kudo, I. (2004) Biol. Pharm. Bull. 27:1158.
  4. de Beer, F. C. and Webb, N. R. (2006) Arterioscler. Thromb. Vasc. Biol. 26:1421.
  5. Wootton, P. T. E. et al. (2006) Human Mol. Genet. 15:355.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Applications: WB, Simple Western, IHC-P
Species: Hu
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
Species: Hu, Mu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Mu
Applications: Enzyme Activity

Publications for PLA2G2A (4925-PL) (0)

There are no publications for PLA2G2A (4925-PL).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PLA2G2A (4925-PL) (0)

There are no reviews for PLA2G2A (4925-PL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for PLA2G2A (4925-PL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

COX-2 Antibody
IL-6 [Unconjugated]

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse PLA2G2A Protein, CF and receive a gift card or discount.


Gene Symbol Pla2g2a