Recombinant Mouse Notum Protein, CF

RecombinantMouse Wnt-3a shows a dose responsive increase in Wnt reporter activity inHEK293 cells (green line). Recombinant Mouse Notum (Catalog #9150-NO)inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN) . The ED50 is 0.2-1.2 µg/mL (orange line).

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Notum Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.2-1.2 μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
• Source: Mouse myeloma cell line, NS0-derived mouse Notum protein
  Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag
• Accession #: Q8R116
• N-terminal Sequence: Ser88, Ser239
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-46 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Notum Protein, CF

• EC 3.-
• notum pectinacetylesterase homolog (Drosophila)
• Notum
• protein notum homolog

Background

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.

1. Zhang, X. et al. (2015) Dev. Cell 32:719.
2. Kakugawa, S. et al. (2015) Nature 519:187.
3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
4. Traister, A. et al. (2008) Biochem. J. 410:503.
5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
7. De Robertis, M. et al. (2015) Oncotarget 6:41237.

Publications for NOTUM (9150-NO)(2)

Publications using 9150-NO

• Wu, X;Yang, X;Dai, X;Chen, X;Shen, M;Dai, J;Yuan, F;Wang, L;Yuan, Y;Feng, Y; 5-Aza-2'-Deoxycytidine Ameliorates Choroidal Neovascularization by Inhibiting the Wnt/?-Catenin Signaling Pathway Investigative ophthalmology & visual science 2024-02-01 [PMID: 38345554] (Bioassay, Mouse)
• D Mizrak, NS Bayin, J Yuan, Z Liu, RM Suciu, MJ Niphakis, N Ngo, KM Lum, BF Cravatt, AL Joyner, PA Sims Single-Cell Profiling and SCOPE-Seq Reveal Lineage Dynamics of Adult Ventricular-Subventricular Zone Neurogenesis and NOTUM as a Key Regulator Cell Rep, 2020-06-23;31(12):107805. 2020-06-23 [PMID: 32579931] (Bioassay, Mouse)

FAQs for NOTUM (9150-NO). (Showing 1 - 1 of 1 FAQs).

1.  is there information available to know if Novus item NBP2-66631 binds to R&D item 9150-NO-050? I am looking to build a sandwich ELISA and am on the hunt for potential antibody pairs and a standard material
   • So while we have not used NBP2-66631 explicitly with 9150-NO-050, it should work to detect this product as the AB was raised against recombinante full length Mouse Notum protein, and R&D's protein is Ser88-Thr458 (Cys337Ser) with a C-terminal 6-His tag. As a polyclonal AB and mouse reactive, this should be a good product to consider. However, we have not validated this AB for use in a sandwich ELISA, so it is not covered in our guarantee.

Bioinformatics

• Uniprot:
  • Q8R116()