Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. 1 μg/mL of Recombinant Mouse IL-28 R alpha /IFN-lambda R1 Fc Chimera was mixed with serially diluted Recombinant Mouse (rm) IL-28B/IFN-lambda 3 (Catalog # 1789-ML). Following incubation, the IL-28 R alpha rmIL-28B complex was captured in a goat anti-mFc-coated plate. Bound IL-28B was measured using biotinylated goat anti-mIL-28B. The concentration of rmIL-28B that produces 50% of the optimal binding was found to be approximately 0.7-3.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-28 R alpha/IFN-lambda R1 protein
  

  Mouse IL-28 R alpha (Arg21-Ala227) Accession # AAH57856	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: AAH57856
• N-terminal Sequence: Arg21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ifnlr1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF

• class II cytokine receptor CRF2/12
• CRF2/12
• CRF2-12
• Cytokine receptor class-II member 12
• Cytokine receptor family 2 member 12
• IFN-lambda R1
• IFN-lambda receptor 1
• IFN-lambda-R1
• IFNLR
• IFNLR1
• IL-28 R alpha
• IL-28 receptor subunit alpha
• IL28R alpha
• IL-28R1
• IL28RA
• IL-28Ra
• IL-28R-alpha
• Interferon lambda receptor 1
• interferon lambda, receptor 1
• interleukin 28 receptor A
• interleukin 28 receptor, alpha (interferon, lambda receptor)
• interleukin 28 receptor, alpha
• interleukin or cytokine receptor 2
• interleukin-28 receptor subunit alpha
• LICR2
• Likely interleukin or cytokine receptor 2

Background

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta , CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1). Mouse IL 28A and IL-28B share 97% amino acid (aa) identity; rodents do not express IL-29 (1-4). Mouse IL-28 R alpha cDNA encodes a 535 aa protein with a 20 aa signal peptide, a 207 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 287 aa cytoplasmic domain. A 254 aa splice variant of mouse IL 28 R alpha diverges at aa 224 and lacks a transmembrane sequence (3, 5). The mature mouse IL-28 R alpha ECD shares 83%, 67%, 64% and 63% aa sequence identity with rat, human, canine and bovine IL-28 Ra, respectively. Mouse IL-28 R will respond to human IL-28A (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs and involve tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for IL-28/IL-28 R (7).

1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
2. Meager, A. et al. (2005) Cytokine 31:109.
3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
5. Entrez Accession # EDL29971.
6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

Bioinformatics

• Gene Symbol: Ifnlr1
• Uniprot:
  • AAH57856()