Recombinant Mouse IL-22 R alpha 1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-22 R alpha 1 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 3-10 µg/mL in the presence of 1 ng/mL of rmIL-22.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-22 R alpha 1 protein
  Thr18-Ala228, with a C-terminal 6-His tag
• Accession #: NP_839988
• N-terminal Sequence: Thr18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il22ra1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-22 R alpha 1 Protein, CF

• CRF2-9
• CRF2-9interleukin 22 receptor
• Cytokine receptor class-II member 9
• Cytokine receptor family 2 member 9
• IL-22 R alpha 1
• IL-22 receptor subunit alpha-1
• IL22R alpha 1
• IL22R
• IL22R1
• IL22RA1
• IL-22Ra1
• IL-22R-alpha-1
• IL-TIF-R1
• interleukin 22 receptor, alpha 1
• interleukin-22 receptor subunit alpha-1
• zcytoR11

Background

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20% - 26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL-22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6 - 9). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11 - 14). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
3. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335. 
4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
14. Wolk, K. et al. (2004) Immunity 21:241.
15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Publications for IL-22R alpha 1 (4294-MR)(1)

Publication using 4294-MR

• Weathington N, Snavely C, Chen B, Zhao J, Zhao Y, Mallampalli R Glycogen synthase kinase-3beta stabilizes the interleukin (IL)-22 receptor from proteasomal degradation in murine lung epithelia. J Biol Chem, 2014-04-17;289(25):17610-9. 2014-04-17 [PMID: 24742671] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Il22ra1
• Uniprot:
  • NP_839988()