Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ICAM-5 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to recombinant mouse ICAM-5 Fc Chimera coated plates (25 µg/mL with 100 µL/well), >25% cells will adhere after 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse ICAM-5 protein
  

  Mouse ICAM-5 Leu31-Arg828 (Pro47Arg) Accession # Q60625.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q60625.1
• N-terminal Sequence: Leu31
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Icam5
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 112 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 166 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

• ICAM5
• ICAM-5
• intercellular adhesion molecule 5
• intercellular adhesion molecule 5, telencephalin
• Telencephalin
• TLCNTelencephalin
• TLNtelencephalin

Background

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38 - 55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N‑glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1).

As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM-5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4 - 5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

1. Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
2. Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
3. Tian, L. et al. (2000) J. Cell Biol. 150:243.

Publications for ICAM-5 (1173-M5)(5)

Publications using 1173-M5

• S Paetau, T Rolova, L Ning, CG Gahmberg Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia Front Mol Neurosci, 2017-12-22;10(0):431. 2017-12-22 [PMID: 29311819] (Bioassay, Mouse)
• Furutani Y, Kawasaki M, Matsuno H, Mitsui S, Mori K, Yoshihara Y Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin. J Biol Chem, 2012-09-27;287(46):39041-9. 2012-09-27 [PMID: 23019340] (Surface Plasmon Resonance)
• Niedringhaus M, Chen X, Dzakpasu R, Conant K MMPs and soluble ICAM-5 increase neuronal excitability within in vitro networks of hippocampal neurons. PLoS ONE, 2012-08-13;7(8):e42631. 2012-08-13 [PMID: 22912716] (Bioassay, Rat)
• Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5. J. Neurochem., 2011-01-19;118(4):521-32. 2011-01-19 [PMID: 21166806] (Bioassay, Rat)
• Conant K, Wang Y, Szklarczyk A, Dudak A, Mattson MP, Lim ST Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation. Neuroscience, 2010-01-04;166(2):508-21. 2010-01-04 [PMID: 20045450] (Enzyme Assay Substrate)

Bioinformatics

• Gene Symbol: Icam5
• Uniprot:
  • Q60625.1()