Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

Images

 
There are currently no images for .

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Mouse ICAM-5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant mouse ICAM-5 Fc Chimera coated plates (25 µg/mL with 100 µL/well), >25% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived
Mouse ICAM-5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu31
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Icam5
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
112 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
166 kDa, reducing conditions
Publications
Read Publications using
1173-M5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

  • ICAM5
  • ICAM-5
  • intercellular adhesion molecule 5
  • intercellular adhesion molecule 5, telencephalin
  • Telencephalin
  • TLCNTelencephalin
  • TLNtelencephalin

Background

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38 - 55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N‑glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1).

As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM-5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4 - 5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

Customers Who Viewed This Item Also Viewed...

NB200-610
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
NB300-749
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
796-IC
Species: Mu
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-46010
Species: Hu, Ca, Mk
Applications: WB, IHC, IF
AF7179
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-31553
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-32875
Species: Hu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF7179
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready
NBP2-16396
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB244
Species: Hu
Applications: WB
NBP2-57496
Species: Hu
Applications: ICC/IF
NBP2-20866
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-P
NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC, IF
1173-M5
Species: Mu
Applications: BA

Publications for (4)

We have publications tested in 2 confirmed species: Rat, N/A.

We have publications tested in 3 applications: Bioassay, Enzyme Substrate, Surface Plasmon Resonance.


Filter By Application
Bioassay
(2)
Enzyme Substrate
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Rat
(2)
N/A
(2)
All Species

Reviews for ICAM-5 (0)

There are no reviews for ICAM-5. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for ICAM-5 (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

ICAM-1/CD54
796-IC-050

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse ICAM-5 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Icam5
Entrez
Uniprot