Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

When Recombinant Mouse GFR alpha ‑like Fc Chimera (Catalog # 9844-GR) is immobilized at 0.1 μg/mL, 100 μL/well, It binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse GFR alpha ‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Biotinylated Recombinant Mouse GDF-15 with an ED₅₀ of 0.3-1.8 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse GFR alpha-like protein
  

  Mouse GFR alpha-like (Gln20-Gly349) Accession # Q6SJE0-1	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q6SJE0-1
• N-terminal Sequence: No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Thr21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

• bA360D14.1
• C6orf144
• GDNF family receptor alpha-like
• GFR alpha like
• GFR alpha-like
• GFRAL
• GRAL
• IVFI9356
• UNQ9356

Background

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

1. Li, Z. et al. (2005) J. Neurochem. 95:361.
2. Yang, L. et al. (2017) Nature Medicine 23:1158.
3. Mullican, S. et al. (2017) Nature medicine 23:1150.

4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Publications for GFR alpha-like (9844-GR)(1)

Publication using 9844-GR

• Beom Yong Lee, Jongwon Jeong, Inseong Jung, Hanchae Cho, Dokyung Jung, Jiwon Shin et al. GDNF family receptor alpha‐like antagonist antibody alleviates chemotherapy‐induced cachexia in melanoma‐bearing mice Journal of Cachexia, Sarcopenia and Muscle 2023-06-01 [PMID: 37017344] (Immunoassay Development, N/A)

Bioinformatics

• Uniprot:
  • Q6SJE0-1(Rat)