Recombinant Mouse BTNL4 His-tag Protein, CF

Recombinant Mouse BTNL4 (Catalog # 9594-BT) inhibitsIL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50for this effect is 0.8-4 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BTNL4 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.8-4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse BTNL4 protein
  Gln29-Trp250, with a C-terminal 6-His tag
• Accession #: NP_109671
• N-terminal Sequence: No results obtained. Gln29 inferred from enzymatic pyroglutamate treatment revealing Glu30
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-39 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BTNL4 His-tag Protein, CF

• BTN3A3
• BTNL4
• EG632126
• NG11

Background

Butyrophilin-like 4, also known as BTLN4, BTN3A3 (human) and BTN3A2 (rat) is an approximately 65 kDa member of the butyrophilin family that may play a role in immune regulation (1-3). The mouse BTNL4 is a 586 aa type 1 transmembrane protein, consisting of a 28 aa signal peptide, followed by a 109 aa immunoglobulin (Ig) V domain, a 71 aa IgC domain, a 20 aa transmembrane domain, and a 170 aa cytoplasmic B30.2/SPRY domain (4). Within the extracellular domain including aa 29‑250, mouse BTNL4 shares 45% and 82% sequence identity with human BTN3A3 and rat BTN3A2, respectively. BTNL4 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL4 co-inhibited anti-CD3 induced IL‑2 secretion on CD3⁺ cells.

1. Yamashiro, H. et al. (2010) J. Leukoc Biol. 88:757.
2. Compte, E. et al. (2004) Eur. J. Immunol. 34:2089.
3. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
4. Bas, A. et al. (2011) Proc Natl Acad Sci U S A. 108:4376.

Bioinformatics

• Uniprot:
  • NP_109671()