Recombinant Human UBE2K/E2-25K Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human UBE2K/E2-25K Protein, CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2K/E2-25K concentration of 0.1-1 μM.
• Source: E. coli-derived human UBE2K/E2-25K protein
  Met1 - Asn200
• Accession #: P61086.3
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2K
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl and TCEP.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UBE2K/E2-25K Protein, CF

• E2(25K)
• E225K
• E2-25K
• EC 6.3.2.19
• Hip2
• HIP-2
• huntingtin interacting protein 2
• Huntingtin-interacting protein 2
• HYPG
• LIG
• UbcH1
• UBE2K
• UBE2K-25K
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 K
• Ubiquitin-conjugating enzyme E2(25K)
• Ubiquitin-conjugating enzyme E2-25 kDa
• Ubiquitin-conjugating enzyme E2-25K
• ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• ubiquitin-protein ligase

Background

Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as E2-25K, HIP2, and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UBE2K share 100% aa sequence identity. Similar to other Ubiquitin-conjugating (E2) enzymes, UBE2K/E2-25K contains a conserved Ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent Ubiquitin binding surface termed the Ubiquitin-associated domain between aa residues 160 and 200 (1,2). Recombinant Human UBE2K/E2-25K is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins; UBE2K/E2-25K mediates the elongation of Lys48-linked poly-Ubiquitin chains (3). UBE2K/E2-25K catalytic activity can be modulated by the post-translational addition of SUMO at Lys14 (4). Substrates include the Huntingtin protein and the tumor suppressor RB1 (5,6). UBE2K/E2-25K is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UBE2K/E2-25K is implicated in polyglutamine diseases and Alzheimer's disease (7-9).

1. Wilson, R.C. et al. (2011) Biochem. Biophys. Res. Commun. 405:662.
2. Haldeman, M.T. et al. (1997) Biochemistry 36:10526.
3. Chen, Z.J. et al. (1991) J. Biol. Chem. 266:15698.
4. Pickler, A. et al. (2005) Nat. Struct. Mol. Biol. 12:264.
5. Kalchman, M.A. et al. (1996) J. Biol. Chem. 271:19385.
6. Oh, K.J. et al. (2010) Virology 396:118.
7. de Pril, R. et al. (2007) Mol. Cell. Neurosci. 34:10.
8. Skibinski, G.A. & L. Boyd (2012) BMC Cell Biol. 13:10.
9. Song, S. et al. (2003) Mol. Cell 12:553.

Publications for UBE2K/E2-25K (E2-603)(2)

Publications using E2-603

• Liu, J;Ackermann, L;Hoffmann, S;Gál, Z;Hendriks, I;Jain, C;Morlot, L;Tatham, M;McLelland, G;Hay, R;Nielsen, M;Brummelkamp, T;Haahr, P;Mailand, N; Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation Nature Structural & Molecular Biology 2024-04-22 [PMID: 38649616] (Bioassay, N/A)
• Y Akizuki, M Morita, Y Mori, A Kaiho-Soma, S Dixit, A Endo, M Shimogawa, G Hayashi, M Naito, A Okamoto, K Tanaka, Y Saeki, F Ohtake cIAP1-based degraders induce degradation via branched ubiquitin architectures Nature Chemical Biology, 2022-10-31;0(0):. 2022-10-31 [PMID: 36316570] (Bioassay, Human)

Bioinformatics

• Gene Symbol: UBE2K
• Uniprot:
  • P61086.3()