Recombinant Human TSPAN14-LEL Fc Chimera Protein, CF

2 μg/lane of Recombinant Human TSPAN14-LEL Fc Chimera Protein (Catalog # 11552-TS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-54 kDa and 90-110 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TSPAN14-LEL Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human TSPAN14 protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human TSPAN14-LEL (LEU114-Asn232) Accession # Q8NG11.1
  N-terminus			C-terminus

• N-terminal Sequence: Met
• Structure / Form: Disulfide linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 43-54 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSPAN14-LEL Fc Chimera Protein, CF

• DC-TM4F2
• tetraspanin 14
• Tetraspanin-14
• TM4SF14
• transmembrane 4 superfamily member 14
• TSPAN14

Background

Tetraspanin 14, or TSPAN14, belongs to a superfamily of proteins that is characterized by four transmembrane domains, three intracellular domains and two extracellular loops: a small extracellular loop (SEL) and a large extracellular loop (LEL). The extracellular loops form molecular webs that bring together cell surface proteins, facilitating the formation of stable and functional signalling complexes. Tetraspanins form microdomains on the plasma membrane that mediate diverse biological processes including adhesion, cell fusion, immune response, and tumor development (1-4). Human TSPAN14 consists of 270 amino acids, with the LEL region spanning residues 114-232. Within the LEL, human TSPAN14 shares 98% aa sequence identity with mouse and rat TSPAN14-LEL.

1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
2. Yang, J. et al. (2024) Cell. 13:193.
3. Hemler, M.E. (2005) Nat. Rev. Mol. Cell Biol.6:801.
4. Kim, T-K. et al. (2015) Biochem. Biophys. Res. Commun. 468:774.