Recombinant Human TAFA2/FAM19A2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TAFA2/FAM19A2 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rhTAFA2, immobilized at 6-24 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
• Source: E. coli-derived human TAFA2/FAM19A2 protein
  Ala31-His131
• Accession #: AAH28403
• N-terminal Sequence: Ala31
• Protein/Peptide Type: Recombinant Proteins
• Gene: FAM19A2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 11.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 11 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TAFA2/FAM19A2 Protein, CF

• Chemokine-like protein TAFA-2
• DKFZp761E1217
• DKFZp781P0552
• FAM19A2
• family with sequence similarity 19 (chemokine (C-C motif)-like), member A2
• MGC42403
• TAFA2
• TAFA-2

Background

TAFA2 (also FAM19A2) is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain (SwissProt #: Q8N3H0). Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX₇CCX₁₃CXCX₁₄CX₁₁CX₄CX₅CX₁₀C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid (1). Human TAFA2 is 97% aa identical to mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000-fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10-fold more abundantly expressed than in other cortical regions) and medulla (1). The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1 - 2). Finally, TAFAs may control axonal sprouting following brain injury (1).

1. Tang, Y.T. et al. (2004) Genomics 83:727.
2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Publications for TAFA2/FAM19A2 (4179-TA)(2)

Publications using 4179-TA

• A Jafari, A Isa, L Chen, N Ditzel, W Zaher, L Harkness, HE Johnsen, BM Abdallah, C Clausen, M Kassem TAFA2 induces skeletal (stromal) stem cell migration through activation of Rac1-p38 signaling Stem Cells, 2018-12-17;0(0):. 2018-12-17 [PMID: 30485583] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)

Bioinformatics

• Gene Symbol: FAM19A2
• Uniprot:
  • AAH28403(Human)