Recombinant Human Ninjurin-2 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Ninjurin-2 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived human Ninjurin-2 protein
  Met1-Thr65, wtih a C-terminal 6-His tag
• Accession #: Q9NZG7
• N-terminal Sequence: Met1
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 8 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ninjurin-2 Protein, CF

• FLJ56349
• Nerve injury-induced protein 2
• NINJ2
• ninjurin 2
• Ninjurin2
• Ninjurin-2

Background

Ninjurin-2 (nerve injury-induced protein 2) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins (1). It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding (1). Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1‑65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128‑142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1‑65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2. Multiple studies have linked NINJ-2 gene polymorphism with ischemic stroke (2-4). In endothelia cells, NINJ-2 activates TLR4 signaling pathway and promote inflammation (5).

1. Araki, T. and J. Milbrandt (2000) J. Neurosci. 20:187.
2. Zhu, Y. et al. (2014) Thromb Thrombolysis. 38:470.
3. Bis. JC. et al. (2014) PLoS One 9:e99798.
4. Li, BH., et al. (2012) J Neurol Sci 316:116.
5. Wang, J. et al. (2017) Cell Signal 35:231.

Bioinformatics

• Uniprot:
  • Q9NZG7()