Recombinant Human NGL-1/LRRC4C Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human NGL-1/LRRC4C Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhNGL-1 at 2 µg/mL can bind rmNetrin-G1a (Catalog # 1166-NG) with apparent K_D < 10 nM.
• Source: Mouse myeloma cell line, NS0-derived human NGL-1/LRRC4C protein
  Gln45-Lys527, with a C-terminal 6-His tag
• Accession #: NP_065980
• N-terminal Sequence: No results obtained: Gln45 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRRC4C
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 54.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NGL-1/LRRC4C Protein, CF

• KIAA1580NGL-1NGL1Netrin-G1 ligand
• leucine rich repeat containing 4C
• leucine-rich repeat-containing protein 4C
• LRRC4C
• NGL1
• NGL-1

Background

Human NGL-1 (Netrin-G1 ligand) is a 67 kDa (predicted for mature protein), type I transmembrane cell adhesion molecule that is a member of the NGL family of proteins (1 - 2). It is synthesized from a precursor that is 640 amino acids (aa) in length that contains a 44 aa signal sequence, a 483 aa extracellular region, a 21 aa transmembrane region, and a short cytoplasmic tail of 92 aa. The extracellular region of NGL-1 consists of nine leucine-rich repeats (LRRs) that are flanked by LRR N-terminal and LRR C-terminal domains, and followed by an Ig-like C2-type domain (1 - 2). The cytoplasmic region contains a C-terminal Glu-Thr-Gln-Ile sequence that corresponds to a potential PDZ (postsynaptic density-95/discs large/zona occludens-1) domain-binding motif (1 - 2). Human NGL-1 is 99.7% aa identical to mouse NGL-1. Mouse NGL-1 is highly expressed in the developing cerebral cortex and the striatum at embryonic day 14 (1). Postnatally, NGL-1 is expressed exclusively in the brain, with the highest expression found in the cerebral cortex as a whole, and in individual neocortical areas such as the frontal, parietal and occipital lobes (1). Moderate expression of NGL-1 occurs in the putamen, amygdala, hippocampus and medulla oblongata (1). Weak expression is found in the caudate nucleus and thalamus (1). Functionally, membrane-bound cell-surface NGL-1 binds to netrin-G1 specifically through its LRR region, and in the developing brain, may promote neurite outgrowth of thalamocortical axons (1 - 4). Little is known about NGL-1’s function at later stages.

1. Lin, J.C. et al. (2003) Nat. Neurosci. 6:1270.
2. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
3. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
4. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14801.

Bioinformatics

• Gene Symbol: LRRC4C
• Uniprot:
  • NP_065980()