Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Latrophilin 3 Protein Fc Chimera, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Latrophilin 3/LPHN3 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human FLRT3 (Catalog # 2795-FL) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL
• Source: Chinese Hamster Ovary cell line, CHO-derived human Latrophilin 3/LPHN3 protein
  

  Human Latrophilin 3 (Phe20-Leu854) Accession # NP_056051	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_056051.2
• N-terminal Sequence: Phe20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ADGRL3
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

• CIRL3
• Latrophilin 3
• latrophilin homolog 3 (cow)
• LEC3
• Lectomedin-3
• LPHN3

Background

Latrophilin-3 (LPHN3) is a 7-transmembrane protein in the family of adhesion GPCRs with large N-terminal extracellular regions (1, 2). The 847 amino acid (aa)
N-terminal region of human Latrophilin-3 contains a SUEL-like lectin domain, an Olfactomedin-like domain, an O-glycosylated mucin-like region, a hormone receptor domain, and a GPCR proteolysis site (GPS). Within this approximately 120 kDa region, human Latrophilin-3 shares 96% aa sequence identity with mouse and rat Latrophilin-3. Alternative splicing of human Latrophilin-3 generates additional isoforms with a 13 aa insertion or premature truncation in the N-terminal ECD (3). Latrophilin-3 is expressed in the brain cortex (3). It binds to FLRT1, 2, and 3 and Tenurin 1 (4, 5). Its binding in trans to post-synaptic FLRT3 at glutamatergic synapses supports the maintenance of excitatory synapses as well as the amplitude of glutamatergic transmission (4, 5). Polymorphisms of Latrophilin-3 are associated with susceptibility to attention deficit/hyperactivity disorder (ADHD) (3, 6). Latrophilin-3 is also expressed in fetal adrenal glands and is down-regulated in the adult (7).

1. Promel, S. et al. (2012) Cell Rep. 2:321.
2. Matsushita, H. et al. (1999) FEBS Lett. 443:348.
3. Arcos-Burgos, M. et al. (2010) Mol. Pschiatry 15:1053.
4. O’Sullivan, M.L. et al. (2012) Neuron 73:903.
5. O’Sullivan, M.L. et al. (2014) Neural Dev. 9:7.
6. Franke, B. et al. (2012) Mol. Psychiatry 17:960.
7. Xing, Y. et al. (2009) Mol. Cell. Endocrinol. 300:43.

Bioinformatics

• Gene Symbol: ADGRL3
• Uniprot:
  • NP_056051.2()