Recombinant Human IL-22 R alpha 1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-22 R alpha 1 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 1-4 µg/mL in the presence of 1 ng/mL of recombinant human IL-22.
• Source: Mouse myeloma cell line, NS0-derived human IL-22 R alpha 1 protein
  Pro18-Thr228, with a C-terminal 6-His tag
• Accession #: NP_067081
• N-terminal Sequence: Pro18
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL22RA1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-22 R alpha 1 Protein, CF

• CRF2-9
• CRF2-9interleukin 22 receptor
• Cytokine receptor class-II member 9
• Cytokine receptor family 2 member 9
• IL-22 R alpha 1
• IL-22 receptor subunit alpha-1
• IL22R alpha 1
• IL22R
• IL22R1
• IL22RA1
• IL-22Ra1
• IL-22R-alpha-1
• IL-TIF-R1
• interleukin 22 receptor, alpha 1
• interleukin-22 receptor subunit alpha-1
• zcytoR11

Background

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF).
IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding
IL-22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10 - 13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
13. Wolk, K. et al. (2004) Immunity 21:241.
14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Publications for IL-22R alpha 1 (2770-LR)(1)

Publication using 2770-LR

• S La Manna, PL Scognamigl, C Di Natale, M Leone, FA Mercurio, AM Malfitano, F Cianfarani, S Madonna, S Caravella, C Albanesi, E Novellino, D Marasco Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces Biochimie, 2017-05-04;0(0):. 2017-05-04 [PMID: 28479106] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: IL22RA1
• Uniprot:
  • NP_067081()