Recombinant Human ICAM-5 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ICAM-5 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human ICAM-5 protein
  

  Human ICAM-5 (Ala28-Glu570) Accession # Q9UMF0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9UMF0
• N-terminal Sequence: Ala28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ICAM5
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 85.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-5 Fc Chimera Protein, CF

• ICAM5
• ICAM-5
• intercellular adhesion molecule 5
• intercellular adhesion molecule 5, telencephalin
• Telencephalin
• TLCNTelencephalin
• TLNtelencephalin

Background

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.

The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
2. Yoshihara, Y. et al. (1994) Neuron 12:541.
3. Tain, L. et al. (1997) J. Immunol. 158:928.
4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
5. Tain, L. et al. (2000) J. Immunol. 150:243.

Publications for ICAM-5 (1950-M5)(2)

Publications using 1950-M5

• J Baggen, Y Liu, H Lyoo, ALW van Vliet, M Wahedi, JW de Bruin, RW Roberts, P Overduin, A Meijer, MG Rossmann, HJ Thibaut, FJM van Kuppev Bypassing pan-enterovirus host factor PLA2G16 Nat Commun, 2019-07-18;10(1):3171. 2019-07-18 [PMID: 31320648] (Bioassay, Virus)
• Wei Wei ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68 Cell Host Microbe, 2016-10-27;20(5):631-641. 2016-10-27 [PMID: 27923705] (Bioassay)

Bioinformatics

• Gene Symbol: ICAM5
• Uniprot:
  • Q9UMF0()