Recombinant Human GPR37 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human GPR37 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human GPR37 protein
  Leu28-Met265, with a C-terminal 6-His tag
• Accession #: O15354
• N-terminal Sequence: Leu28
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37-53 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GPR37 Protein, CF

• EDNRBL
• Endothelin B receptor-like protein 1
• ETBR-LP-1
• G protein-coupled receptor 37 (endothelin receptor type B-like)
• GPR37
• hET(B)R-LP
• PAELR
• PAELRETBR-LP-1
• Parkin-associated endothelin receptor-like receptor
• probable G-protein coupled receptor 37

Background

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin  (1, 4).  It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus (2). It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress (3). In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death (2). The  extracelluar domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.

1. Marazziti, D. et al. (1997) Genomics, 45:68.
2. Donohue, P.J. et al. (1998) Brain Res Mol Brain Res. 54:152.
3. Omura, T. et al. (2006) J Neurochem. 99:1456.
4. Meyer, R.C. et al. (2013) Proc. Natl. Acad. Sci. U. S. A. 110: 9529

 

Bioinformatics

• Uniprot:
  • O15354()