Recombinant Human GFR alpha-like His-tag Protein, CF

When Recombinant Human GFR alpha ‑like (Catalog # 9647‑GR) is immobilizedat 0.1 μg/mL, 100 μL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279‑GD) with an ED50 of 0.15‑0.9 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GFR alpha-like His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15 (Catalog # 9279-GD) with an ED₅₀ of 0.15-0.9 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human GFR alpha-like protein
  Ser19-Glu351, with a C-terminal 6-His tag
• Accession #: Q6UXV0
• N-terminal Sequence: Ser19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37-54 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GFR alpha-like His-tag Protein, CF

• bA360D14.1
• C6orf144
• GDNF family receptor alpha-like
• GFR alpha like
• GFR alpha-like
• GFRAL
• GRAL
• IVFI9356
• UNQ9356

Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively.  GFRAL is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

1. Li, Z. et al. (2005) J. Neurochem. 95:361.
2. Mullican, S. et al. (2017) Nat. Med 23:1150.
3. Yang, L. et al. (2017) Nat. Med 23:1158.
4. Emmerson, P. et al. (2017) Nat. Med 23:1215.

Publications for GFR alpha-like (9647-GR)(2)

Publications using 9647-GR

• Xie, B;Tang, W;Wen, S;Chen, F;Yang, C;Wang, M;Yang, Y;Liang, W; GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex Biomolecules 2023-12-27 [PMID: 38254638] (Bioassay, Human)
• Alexopoulou, F;Buch-Månson, N;Pedersen, SL;Vrang, N;Fink, LN;Strømgaard, K; Identification of GDF15 peptide fragments inhibiting GFRAL receptor signaling Peptides 2023-07-24 [PMID: 37495041] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q6UXV0()