Recombinant Human GASP-1/WFIKKN2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity, Inhibition Activity
• Format: Carrier-Free

Recombinant Human GASP-1/WFIKKN2 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. The ED₅₀ for this effect is 0.03-0.15 µg/mL, in the presence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog # ES002). The IC₅₀ value is approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nM trypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl₂, 0.15 M NaCl, pH 7.5.
• Source: Mouse myeloma cell line, NS0-derived human GASP-1/WFIKKN2 protein
  Leu35-His576, with a C-terminal 6-His tag
• Accession #: Q8TEU8
• N-terminal Sequence: Leu35
• Protein/Peptide Type: Recombinant Proteins
• Gene: WFIKKN2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Inhibition Activity
• Theoretical MW: 60.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GASP-1/WFIKKN2 Protein, CF

• GASP1
• GASP-1
• Growth and differentiation factor-associated serum protein 1
• hGASP-1
• WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein
• WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
• WAP, FS, Ig, two KU and NTR module related protein
• WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2
• WFIKKN2
• WFIKKN-related protein
• WFIKKNRP
• WFIKKNRPmultivalent protease inhibitor protein

Background

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP/WFIKKN2 and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP/WFIKKN2. WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP-2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.

1. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
2. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
3. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
4. Trexler, M. et al. (2002) Biol. Chem. 383:223.

Publications for GASP-1/WFIKKN2 (2070-GS/CF)(2)

Publications using 2070-GS/CF

• RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson Structural basis for potency differences between GDF8 and GDF11 BMC Biol, 2017-03-03;15(1):19. 2017-03-03 [PMID: 28257634] (Bioassay, Human)
• Bonala S, Lokireddy S, Arigela H, Teng S, Wahli W, Sharma M, McFarlane C, Kambadur R Peroxisome proliferator-activated receptor beta/delta induces myogenesis by modulating myostatin activity. J. Biol. Chem., 2012-02-23;287(16):12935-51. 2012-02-23 [PMID: 22362769] (Bioassay, Human)

Bioinformatics

• Gene Symbol: WFIKKN2
• Uniprot:
  • Q8TEU8()