Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF


There are currently no images for Carboxypeptidase A2/CPA2 (2896-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Carboxypeptidase A2/CPA2 protein
Leu17-Tyr417, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
43 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in 25 mM Tris and 150 mM NaCl, pH 7.5.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
  • Recombinant Human Carboxypeptidase A2/CPA2 (rhCPA2) (Catalog # 2896-ZN)
  • Trypsin (Sigma, Catalog # T-1426)
  • Substrate: Ac-Phe-Thiaphe-OH (Peptides International, Catalog # STP-3621-PI),10 mM stock in DMSO
  • 5,5’-dithio-bis(2-nitrobenzoic acid) (DTNB), (Sigma, Catalog # D-8130) 10 mM stock in DMSO
  • 96 well clear plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhCPA2 to 100 µg/mL with 1.0 µg/mL Trypsin in Assay Buffer.
  2. Incubate at room temperature for 60 minutes.
  3. Dilute active rhCPA2 to 0.1 µg/mL in Assay Buffer.
  4. Combine equal volumes of 10 mM Substrate and 10 mM DTNB. Then, dilute this mixture to 200 μM Substrate, 200 μM DTNB with Assay Buffer.
  5. Load 50 µL of the 0.1 µg/mL rhCPA2 into microplate. Include a Substrate Blank with 50 µL of Assay Buffer in place of rhCPA2.
  6. Start the reaction by adding 50 µL of 200 µM Substrate into wells.
  7. Read in kinetic mode for 5 minutes at an absorbance of 405 nm.
  8. Calculate specific activity using the following formula:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/M
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 13260 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhCPA2: 0.005 µg
  • Substrate: 100 µM
  • DTNB: 100 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF

  • carboxypeptidase A2 (pancreatic)
  • Carboxypeptidase A2
  • CPA2
  • EC 3.4.17
  • EC


Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed under the conditions described in the Activity Assay Protocol.

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P, Flow-IC
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Species: Hu
Applications: Flow, CyTOF-ready
Species: Hu, Ca, Mk
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Carboxypeptidase A2/CPA2 (2896-ZN) (0)

There are no publications for Carboxypeptidase A2/CPA2 (2896-ZN).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Carboxypeptidase A2/CPA2 (2896-ZN) (0)

There are no reviews for Carboxypeptidase A2/CPA2 (2896-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Carboxypeptidase A2/CPA2 (2896-ZN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Carboxypeptidase A2/CPA2 (2896-ZN)

Discover related pathways, diseases and genes to Carboxypeptidase A2/CPA2 (2896-ZN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Carboxypeptidase A2/CPA2 (2896-ZN)

Discover more about diseases related to Carboxypeptidase A2/CPA2 (2896-ZN).

Pathways for Carboxypeptidase A2/CPA2 (2896-ZN)

View related products by pathway.

PTMs for Carboxypeptidase A2/CPA2 (2896-ZN)

Learn more about PTMs related to Carboxypeptidase A2/CPA2 (2896-ZN).

Blogs on Carboxypeptidase A2/CPA2

There are no specific blogs for Carboxypeptidase A2/CPA2, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Carboxypeptidase A2/CPA2 Protein, CF and receive a gift card or discount.


Gene Symbol CPA2