Recombinant Human Calsyntenin-1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Calsyntenin-1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED₅₀ for this effect is typically 1-4 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human Calsyntenin-1 protein
  Arg30-Thr851, with a C-terminal 6-His tag
• Accession #: AAH33902
• N-terminal Sequence: Arg30
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLSTN1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 92.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Calsyntenin-1 Protein, CF

• alcadein alpha 1
• Alcadein-alpha
• ALC-ALPHA
• alcalpha1
• alcalpha2
• Alzheimer-related cadherin-like protein
• cadherin-related family member 12
• calsyntenin 1
• Calsyntenin1
• Calsyntenin-1
• CDHR12
• CLSTN1
• CS1
• CSTN1
• FLJ32258
• KIAA0911alcadein-alpha
• Non-classical cadherin XB31alpha
• non-classical cadherin XB31alpha1
• PIK3CD
• XB31alpha

Background

Calsyntenin-1 (CST-1; gene name CLSTN1), also called Alcadein-alpha , is a 140‑150 kDa type I transmembrane glycoprotein that is member of the Alcadein family of cadherin domain-containing molecules (1, 2). The human Calsyntenin-1 cDNA encodes 981 amino acids (aa), including a 28 aa signal sequence and the 953 aa mature protein (1). Further cleavage by ADAM10 and ADAM17 generates an N-terminal 115 kDa portion containing the two cadherin domains (aa 38‑265), termed soluble Alc‑ alpha , and a C-terminal portion, termed CTF1-alpha (1, 3). CTF1-alpha contains 34 extracellular aa, 21 transmembrane aa, and 101 cytoplasmic aa that are highly acidic and bind calcium (1, 4). Isoforms of 962-994 aa contain a 78 aa substitution for aa 917‑981, with or without deletions of aa 72‑81 and 507‑525. Within the soluble Alc‑ alpha portion (aa 30‑851), human Calsyntenin-1 shares 92% aa sequence identity with mouse, 93% with equine, and 91% with rat, canine, bovine and porcine Calsyntentin-1. It is expressed in neuronal post-synaptic plasma membranes, neuronal APP-containing early endosomal vesicles (full-length CST-1) and APP-negative recycling‑endosomal vesicles (CST-1 cleaved form), plus the secretory granules of anterior pituitary basophilic gonadotrophs and pancreatic islet alpha -cells (1-6). Soluble Alc‑ alpha mediates adhesion and can be released from axons, while CTF1-alpha can be internalized and mediates trafficking of vesicles via interaction with Kinesin-1 (1, 6-9). This interaction facilitates intracellular transport of vesicles along microtubules in axonal growth cones (7-10). Calsyntenin regulates amyloid precursor protein (APP) cleavage and transport, and perturbation of axonal transport of APP by CST-1 containing carriers results in increased production of A beta . Finally, CST-1 may also play a regulatory role in post-synaptic signaling (1, 2, 4, 8, 10).

1. Vogt, L. et al. (2001) Mol. Cell. Neurosci. 17:151.
2. Araki, Y. et al. (2003) J. Biol. Chem. 278:49448.
3. Hata, S. et al. (2009) J. Biol. Chem. 284:36024.
4. Hintsch, G. et al. (2002) Mol. Cell. Neurosci. 21:393.
5. Rindler, M.J. et al. (2008) J. HIstochem. Cytochem. 56:381.
6. Steuble, M. et al. (2010) Proteomics 10:3775.
7. Konecna, A. et al. (2006) Mol. Biol. Cell 17:3651.
8. Araki, Y. et al. (2007) EMBO J. 26:1475.
9. Vagnoni, A. et al. (2011) J. Cell Sci. 24:1032.
10. Ludwig, A. et al. (2009) Traffic 10:572.
11. Vagnoni, A. et al. (2012) Hum. Mol. Genet. Mar. 27 [Epub ahead of print].

Bioinformatics

• Gene Symbol: CLSTN1
• Uniprot:
  • AAH33902()