RBMS1 Overexpression Lysate (Native)

Images

 
Western Blot: RBMS1 Overexpression Lysate (Adult Normal) [NBL1-15219] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBMS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RBMS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203437 and based on accession number NM_016836. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RNA binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 1, mRNA.
Gene
RBMS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RBMS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RBMS1 Overexpression Lysate (Native)

  • chromosome 2 open reading frame 12
  • DKFZp564H0764
  • HCC-4
  • MGC15146
  • MGC3331
  • MGC97258
  • MGC97270
  • MGC97282
  • MGC99543
  • MSSP1
  • MSSP-1
  • MSSP-2
  • MSSP-3
  • RNA binding motif, single stranded interacting protein 1
  • SCR2MGC70597
  • single-stranded-interacting protein 1
  • suppressor of cdc 2 (cdc13) with RNA binding motif 2

Background

This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. Several transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. A pseudogene for this locus is found on chromosome 12. [provided by RefSeq]. Transcript Variant: This variant (1), also known as YC1 and MSSP-1, encodes the longest isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RBMS1