Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric)

ELISA: Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) [NBP3-00454] - Samples were spiked with high concentrations of Mouse RAR gamma/NR1B3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) [NBP3-00454] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse RAR gamma/NR1B3 in samples. No significant cross-reactivity or interference between Mouse RAR gamma/NR1B3 and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.16%
• Intra-Assay: 4.6%
• Spike Recovery: 86 - 108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: RARG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse RAR gamma/NR1B3 ELISA Kit (Colorimetric)

• NR1B3
• NR1B3RARC
• Nuclear receptor subfamily 1 group B member 3
• RAR gamma
• RARC
• RARG
• RAR-gamma
• retinoic acid receptor gamma
• retinoic acid receptor, gamma

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters. Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition. While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrett's tissues compared with normal tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for RAR gamma/NR1B3 ELISA Kit (NBP3-00454). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: RARG