RAR alpha/NR1B1 Antibody (SN0725)

Western Blot: RAR alpha/NR1B1 Antibody (SN0725) [NBP2-67712] - Analysis of RAR alpha/NR1B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate

Flow Cytometry: RAR alpha/NR1B1 Antibody (SN0725) [NBP2-67712] - Analysis of MCF-7 cells with RAR-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Discontinued

RAR alpha/NR1B1 Antibody (SN0725) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RAR alpha/NR1B1 aa 1-50 / 462. (SwissProt: P10276 Human; SwissProt: P11416 Mouse)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RARA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:50-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RAR alpha/NR1B1 Antibody (SN0725)

• alpha polypeptide
• NR1B1
• RAR alpha
• RARA
• RAR-alpha
• retinoic acid receptor, alpha

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RARA