RAMP3 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

RAMP3 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human RAMP3
  Arg24-Val118
  Accession # O60896
• Specificity: Detects human RAMP3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RAMP1, rhRAMP2, and recombinant mouse RAMP2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RAMP3 Antibody [Alexa Fluor® 647]

• calcitonin receptor-like receptor activity modifying protein 3
• Calcitonin-receptor-like receptor activity-modifying protein 3
• CRLR activity-modifying protein 3
• RAMP3
• receptor (calcitonin) activity modifying protein 3
• receptor (G protein-coupled) activity modifying protein 3
• receptor activity modifying protein 3
• receptor activity-modifying protein 3
• receptor-activity-modifying protein 3

Background

RAMP3 (receptor activity modifying protein 3) is a 20-26 kDa member of the RAMP family of proteins. It is expressed on multiple cell types in conjunction with CRLR and NHERF-1, thereby generating a receptor complex for adrenomedullin (AM). Mature human RAMP3 is a 125 amino acid (aa) type I transmembrane glycoprotein that contains a 95 aa extracellular domain (ECD) (aa 24-118) and a 10 aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 59-65 are critical for AM binding. There is one potential splice variant that shows a 49 aa substitution for the first 19 aa of the signal sequence. The human ECD is 88% aa identical to the mouse ECD. Members of RAMP family of proteins are known to form complexes with apparent molecular weights of 25 to 50 kDa that are resistant to denaturing and reducing agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.