Rabenosyn 5 Antibody

Western Blot: Rabenosyn 5 Antibody [NB300-813] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rabenosyn 5 Antibody Summary

• Immunogen: Peptide with sequence C-ELKHTLAKQKGGTD corresponding to C-Terminus according to NP_071735.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RBSN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1.5 ug/mL
• Application Notes: Western blot: Approx. 110 kDa band observed in A431, fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa band according to NP_071735). Band migration is consistent with scientific literature (PMID 11062261, 15020713). Knockout validation (PMID: 31988382).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rabenosyn 5 Antibody

• 110 kDa protein
• FLJ34993
• FYVE finger-containing Rab5 effector protein rabenosyn-5
• FYVE-finger-containing Rab5 effector protein rabenosyn-5
• MGC126210
• Rabenosyn-5
• ZFYVE20
• Zinc finger FYVE domain-containing protein 20
• zinc finger, FYVE domain containing 20

Background

DNA binding protein involved in regulation of transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rabenosyn 5 Antibody (NB300-813)(3)

Publications using NB300-813

• Solinger JA, Rashid HO, Prescianotto-Baschong C, Spang A FERARI is required for Rab11-dependent endocytic recycling Nat. Cell Biol. 2020-01-27 [PMID: 31988382] (KO, WB, Human)
• Naslavsky N, Boehm M, Backlund PS Jr et al. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Mol Biol Cell 2004-05-01 [PMID: 15020713]
• Nielsen E, Christoforidis S, Uttenweiler-Joseph S et al. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol 2000-10-30 [PMID: 11062261]

Bioinformatics

• Gene Symbol: RBSN
• Entrez:
  • 64145(Human)
• Uniprot:
  • NP_071735.(Human)