PTH1R/PTHR1 Antibody

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human PTH1 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5709) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PTH1 R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PTH1 R was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human PTH1 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5709) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PTH1R/PTHR1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human PTH1 R
  Tyr23-Met189
  Accession # Q03431
• Specificity: Detects human PTH1 R in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PTH1R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTH1R/PTHR1 Antibody

• MGC138426
• parathyroid hormone 1 receptorMGC138452
• parathyroid hormone/parathyroid hormone-related peptide receptor
• parathyroid hormone/parathyroid hormone-related protein receptor
• PFE
• PTH/PTHr receptor
• PTH/PTHrP type I receptor
• PTH1 receptor
• PTH1R
• PTHR
• PTHR1
• PTHRPTHR1parathyroid hormone receptor 1
• seven transmembrane helix receptor

Background

PTH1 R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66 - 68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca⁺⁺-release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27 - 188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling. Over aa 1 - 189, human PTH1 R shares 88% aa identity with mouse PTH1 R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTH1R/PTHR1 Antibody (AF5709)(1)

Publication using AF5709

• Y Muto, PC Wilson, N Ledru, H Wu, H Dimke, SS Waikar, BD Humphreys Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney Nature Communications, 2021-04-13;12(1):2190. 2021-04-13 [PMID: 33850129] (IHC, Human)

Bioinformatics

• Gene Symbol: PTH1R
• Uniprot:
  • Q03431()