PTGER4/EP4 Overexpression Lysate (Native)

Images

 
Western Blot: Prostaglandin E Receptor EP4 Overexpression Lysate (Adult Normal) [NBL1-14934] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prostaglandin E ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PTGER4/EP4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210932 and based on accession number NM_000958. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens prostaglandin E receptor 4 (subtype EP4) (PTGER4), mRNA.
Gene
PTGER4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PTGER4/EP4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTGER4/EP4 Overexpression Lysate (Native)

  • EP4
  • EP4R
  • MGC126583
  • PGE receptor EP4 subtype
  • PGE receptor, EP4 subtype
  • PGE2 receptor EP4 subtype
  • prostaglandin E receptor 4 (subtype EP4)
  • prostaglandin E2 receptor EP4 subtype
  • Prostanoid EP4 receptor
  • PTGER2
  • PTGER4

Background

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PTGER4/EP4 Lysate (NBL1-14934) (0)

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Video Protocols

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Bioinformatics

Gene Symbol PTGER4
Entrez