PTGER1 Antibody (998412) [Unconjugated]

PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB10008) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 998412
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PTGER1 Antibody (998412) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human PTGER1
  Accession # P34995
• Specificity: Detects human PTGER1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTGER1 Antibody (998412) [Unconjugated]

• EP1
• PGE receptor EP1 subtype
• PGE receptor, EP1 subtype
• PGE2 receptor EP1 subtype
• prostaglandin E receptor 1 (subtype EP1), 42kD
• prostaglandin E receptor 1 (subtype EP1), 42kDa
• prostaglandin E receptor 1, subtype EP1
• prostaglandin E2 receptor EP1 subtype
• Prostanoid EP1 receptor
• PTGER1

Background

Prostaglandin E2 Receptor 1/ PTGER1/EP1, a member of the G protein-coupled receptor family, is one of four receptors for Prostaglandin E2 (PGE2). Expression is highest in kidney and spleen, with very low expression in most other tissues. Stimulation of angiogenesis in primate ovulatory follicles by luteinizing hormone has been shown to be mediated by PGE2 through PTGER1 and PTGER2 receptors. In epidermis, E2 plays a role in keratinocyte differentiaton. Expression and function of EP1 in carcinomas has been studied. For example, cytoplasmic and nuclear localization of EP1 in breast cancer has been reported in association with prognostic markers. And activation of EP1 mediated by COX2 causes VEGFC upregulation in lung adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P34995(Human)